Study on extracellular H+ transport kinetics during oogenesis of red cone

The Journal of Experimental Biology
H + kinetics regulates egg production
Extracellular H + transport kinetics during oogenesis of red cone

Egg production is very important in the process of organism formation, affecting embryonic development and individual formation. In this process, H + kinetics plays a key regulatory role, and cells maintain membrane potential and produce transmembrane through H + transport. The chemical gradient drives secondary metabolism, but little is known about the regulation of H + flux changes during the egg-forming process of insect trophoblasts and the pH changes caused by H + flow. Real-time monitoring of H + flow at different times and spaces can be achieved by using “non-invasive micro-test technology” , thus revealing the regulation effect of H + flow in the process of insect trophic oocyte production.

Bjornsson CS, a scientist at the Center for Neurological Disorders Research at Wadsworth Center in the United States, used "non- invasive micro-measurement technology" to perform real-time monitoring of extracellular H + flow in different growth stages of Rhodius prolixus at the same growth site. The main stem separating the adjacent follicular vesicles in the ovarian tube showed significant H + outflow, while the H + outflow was weakened in the late stage of yolk formation. The phenomenon of H + efflux has not only occurred before the formation of the main stem, but also precedes the initial stage of yolk formation in the adjacent follicular sac. In addition, the outflow of H + was also detected after the end follicles and follicular sacs of the chorion were generated, which revealed that the follicular epithelial cells of the body controlled the outflow of H + .

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Above :
The above figure shows the changes in H + flow detected during different growth stages of different parts of the ovarian tube by non-invasive micro-measurement. Positive values ​​are outflows and negative values ​​are inflows.

This study used non-invasive micro-test technology to detect the change of H + flow during insect egg formation, revealing the importance of H + flow in the initial stage of yolk production, endocytosis, follicular cytoskeletal dynamics and follicular feedback mechanism regulation. Role, transmembrane H + flow can contribute to changes in intracellular pH. This provides novel ideas and methods for the study of the mechanism of insect egg formation.

Key words : H + , ion-selective probe, oogenesis
References : Bjornsson CS, et al. The Journal of Experimental Biology, 2004, 207, 2835-2844
Full text download : http://jeb.biologists.org/cgi/content/abstract/207/16/2835
Abstract:
The spatiotemporal dynamics of transmembrane hydrogen ion (H + ) fluxes During oogenesis were investigated in the telotrophic ovarioles Of the insect, Rhodnius prolixus . Although Rhodnius ovarioles possess Exaggerated morphological and electrical polarity between nurse Cells and oocytes, little is known about H + changes during oogenesis, The regulatory role played by H + and pH in many relevant cellular A number of dynamic extracellular H + fluxes were Between along Rhodnius ovarioles, representing an oogenesis cycle, Using a non-invasive, self-referencing, H + -selective probe. The Interfollicular stalk separating adjacent follicles exhibit Prominent H + efflux that peaked during midvitellogenesis and Revised during late vitellogenesis. H + efflux in this region Preced stalk formation and, importantly, preceded the onset Of vitellogenesis in the adjacent posterior follicle. H + efflux Was also observed over the terminal follicle, where specialized Regions of the chorion were forming, and was still detected Around follicle cells after ovulation, indicating that the somatic Follicular epithelium produced this flux. Transmembrane H + fluxes May drive intracellular pH changes or may stabilize pH i in response To pH-altering events. H + fluxes may play a role in processes That coincide spatially and temporally, including the onset Of vitellogenesis, endocytosis, follicle cell cytoskeletal dynamics, And regulation of interfollicular feedback mechanisms.

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