Different effects of long-string and short-string θ rhythm stimulation on long-term potentiation induced by anterior cingulate cortex in rats: planar microelectrode array recording technique
Summary: Full text download: http:// Manual Seeder,Manual Corn Seeder,Two Barrel Manual Seeder,Agricultural Seeder Machine Henan Qianli Machinery Co.,Ltd. , https://www.chalionagri.com
OBJECTIVE: A large number of studies have shown that anterior cingulate cortex (ACC) is a multifunctional limbic system that participates in a variety of advanced brain functions such as perception, exercise, mood and cognition. There is evidence that long-term potentiation (LTP) is an important model for studying changes in synaptic plasticity in neurons in ACC. However, to date, the temporal and spatial characteristics of ACC neural networks are still less well known. The aim of this study was to investigate the effects of different lengths of θ rhythm string stimulation on LTP induced by different layers of ACC in rats using planar microelectrode array recording technique.
Methods The planar microelectrode array recording technique was used to record on acutely isolated rat ACC brain slices. The field potential of each layer of ACC was recorded by experimentally stimulating the V-VI layer. Then the two θ rhythm stimulation (TBS) was used as the conditional stimulus to induce LTP in the ACC brain region, and the LTP elicitation effects of different layers were compared. The two stimulation mode parameters are: TBS1, 100 Hz, 4 bidirectional square wave pulses are one string, repeat 10 times, interval 200 ms; TBS 2,100 Hz, 4 bidirectional square wave pulses are one string, repeat 5 times, Interval 200 ms. Before the experimental stimulation, the anterior cingulate gyrus can simultaneously induce three different field potentials in the I layer, the II-III layer and the V-VI layer: the I layer is a forward wave and the V-VI layer is a negative wave, II The -III layer is dominated by complex waveforms.
Results After the two different conditional stimuli were given at the experimental stimulation site, the LTP induction rate of long-string TBS1 conditional stimulation was significantly higher than that of short-string TBS2 conditional stimulation, that is, the LTP-induced rate of TBS1 exceeded 60%, while the LTP of TBS2 was The ejaculation rate is less than 25%. In addition, the inter-layer analysis showed that LTP mainly occurred in the II-III layer and the V-VI layer, and the I layer could not induce LTP. Further analysis showed that there was no significant difference in the LTP extraction rate or amplitude between the II-III layer and the V-VI layer.
Conclusions The results of this experiment suggest that the same stimulation pattern but different durations of repeated stimulation may also lead to different LTP induction rates. This may be one of the key reasons why the results of the previous anterior cingulate gyrus LTP seizure rate were different. It is more important to choose the best stimulation parameters for this type of experiment. In addition, this experiment demonstrates that the planar microelectrode array recording technique is used to study the network characteristics of the anterior cingulate cortex synaptic plasticity, especially the spatial and temporal characteristics in different layer structures have the advantage that single electrode can not be compared.
Key words: long-term potentiation; anterior cingulate cortex; θ rhythm stimulation; planar microelectrode array recording technique; rat