Preparation of strains of Stropharia capsula
The production method of Stropharia sp. strain strain is basically the same as the production method of mushrooms and straw mushroom strains. The pure strains can be obtained by tissue separation method and spore separation method. The new area mainly depends on the introduction of the parent species or the original species. The culture media and stock culture materials suitable for the production of the Stropharia capsici parent species are as follows: 1. Mother seed culture medium 1. Maltose yeast agar medium 1 g of soy peptone, 2 g yeast, 20 g maltose, and agar 20 Grams, add water to 1000 ml. 2. Potato potato agar culture medium 300 g potato (water 1500 ml, boiled for 20 minutes, filter juice), yeast 2 g, soybean meal 1 g, glucose 10 g, agar 20 g, add water to 1000 ml. 3. Oatmeal maltose yeast agar medium 80 g oatmeal, 10 g maltose, 2 g yeast, 20 g agar, and water was added to 1000 ml. The above three formulations can be used as a liquid medium without addition of agar. The above medium should be prepared, packed, sterilized, inoculated and cultured as usual. Second, the original species and cultivating species culture material 1, the wheat stalk or naked wheat straw, chopped (2-3 cm long), soaked, bottling, autoclave back. 2, wheat, rye, sorghum, corn, millet and other grains soaked, boiled to no white core but the skin is not broken, add 2% calcium carbonate, bottling, autoclave after use. 3. Use 40% each of fine sawdust and small pieces of wood and 20% of bran to make cultivating culture medium. It can also be inoculated with liquid strains that have been cultured for 3-4 days. If the use of solid strains must increase the inoculum size (strain/culture material), the inoculum size should be at least 10%, preferably 15% to 20%. 4, training. After inoculation, the strainer bottle or bag is cultured in a 20°C-28°C incubator. A few days after the Stropharia mycelium grows, the growth rate of the mycelium gradually becomes slow, and the method of accelerating the growth of the mycelium is stirring. Sterilized wheat medium inoculated with liquid strains is shaken once every 3 to 7 days to shake off the mycelia, which can stimulate the regeneration of hyphae and ensure hypha growth.