Application of in vivo imaging technology in cerebral ischemia research
Application of in vivo imaging technology in cerebral ischemia research Cerebrovascular disease has become an important disease that harms human health all over the world. The use of animal cerebral ischemia and ischemia-reperfusion models to simulate and study human cerebrovascular diseases is a common research tool in current neuroscience. After cerebral ischemia occurs, it is accompanied by the formation of new blood vessels. How to detect the blood flow of new blood vessels and the assessment of the degree of cerebral ischemia are also hot topics in current research. Traditionally used for the diagnosis of ischemic injury in humans, such as magnetic resonance imaging (MRI) and computed tomography (CT), because of its high cost of application and low applicability, it is rarely used for animal cerebral ischemia. Evaluation of the model. In vivo imaging technology uses a fluorescent probe excited by the near-infrared wavelength range, indocyanine green (ICG), to evaluate blood flow and infarct size of brain blood vessels after cerebral ischemia-reperfusion in small animals. Researchers such as Hye-Min Kang from Kyung Hee University School of Medicine in Korea used the VISQUE in vivo imaging system to evaluate changes in meningeal vascular changes, infarct size, and infarcted blood flow in a mouse model of cerebral ischemic injury. After the function recovery has a more intuitive understanding. First, the researchers used the C57 BL/6 mouse to construct a PIO (cerebral ischemic surgery) model, while administering ICG preparations at a dose of 0.001 mg/g body weight, from pre-PIO surgery to 30 days after surgery, in real time. Dynamic kinetic imaging of arterial, venous, and venous sinus in the relevant regions of the brain was observed in the model. The results showed that the vascular function of the brain-related area was severely damaged 1 day after the operation, and the blood flow signal gradually improved after 5 days. After 30 days, the blood circulation of the brain was basically normal (Fig. 1). Figure 1: ICG mode real-time imaging (Time-series) and vascular color imaging Next, the researchers further examined the infarct size of the damaged brain region, and observed the trend of I max using the viscosity analysis software CleVue in VISQUE in vivo imaging. The results were found to be highly consistent with the results of TTC staining (a commonly used indicator of cerebral ischemic injury) (Fig. 2), which also confirms the accuracy of VISQUE in vivo imaging results. Figure 2: In vivo imaging and TTC staining of the infarcted area of ​​the brain Finally, the authors analyzed the blood flow index (BFI) and mean blood flow time (MTT) in the ischemic region of the brain (Fig. 3). The results showed that: 1 day after PIO, the blood circulation in the relevant area was significantly impaired, and the vascular function was significantly restored after 30 days. Figure 3: Comparison of BFI and MTT parameters during postoperative recovery of PIO Current research indicates that the VISQUE in vivo imaging system can observe physiological recovery after cerebral ischemia by real-time imaging in ICG mode. In addition, the image analysis method can also evaluate the efficacy evaluation of drugs related to brain injury. It can be foreseen that in the preclinical study of brain-related diseases such as cerebral ischemia and stroke, the VISQUE living imaging system will become an indispensable scientific research method. references: Kang, HM, I. Sohn, and C. Park, Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains. J Neurosci Methods, 2015. 248 : p. 46-50. Tetanus Vaccine,Hepatitis B Vaccine For Adults,Tetanus Booster,Td Vaccine FOSHAN PHARMA CO., LTD. , https://www.fs-pharma.com
