Development and history of VHP sterilization technology

Development and history of VHP sterilization technology

1 Overview

Vaporized hydrogen peroxide (VHP) sterilization technology is the advantage of using hydrogen peroxide at room temperature to have a more spore-killing ability than a liquid state. After generating free -OH, it is used to attack cellular components, including lipids and proteins. And DNA, a technique that meets the requirements for complete sterilization. It is often used for sterilization of confined spaces such as isolation chambers and isolators.

2, concept

VHP=Vaporized Hydrogen Peroxide(H2O2)

Gasified hydrogen peroxide (H2O2) is commonly known as hydrogen peroxide. Hydrogen peroxide is normally in a liquid state and is heated to become gaseous.

3, VHP history

(1) In the late 1980s, Sterilse Co of the United States first discovered that gaseous hydrogen peroxide can achieve the same sterilization effect with a lower concentration than liquid hydrogen peroxide.

(2) In 1990, gasification hydrogen peroxide officially passed the US EPA, as a sterilizing agent, and was quickly applied in various industrial fields.

4, VHP sterilization principle

(1) Hydrogen peroxide has a bactericidal effect due to its redox effect, and particularly has a good killing effect on anaerobic bacillus.

(2) The principle of action of hydrogen peroxide is to dissociate a highly active hydroxyl group by a complicated chemical reaction and destroy the cell membrane.

5, VHP technical characteristics

(1) Low temperature sterilization process (4-80 ° C).

(2) After the steaming process is completed, there is very little residue (no need to clean again)

(3) No toxic by-products after steaming (health & safety)

(4) No effect on other items (devices, electrical appliances, clean room wall panels, etc.)

(5) VHP sterilization process is very easy to verify (in compliance with regulatory requirements, process control)

(6) Environmental protection (health & safety)

(7) Good penetration of HEPA for high efficiency filters (glass fiber)

(8) It is very effective in sterilizing most microorganisms at low gas concentration (1-2mg/l = 1000ppm)

(9) Short time required for sterilization

(10) Cost saving (short downtime)

6, VHP and various types of sterilization methods

6.1 Status of formaldehyde use

(1) The gas is formed by heating with formaldehyde solution or formalin

(2) Slow effect, need long steaming time

(3) Crystalline residue

(4) No publications have proven effective before and after sterilization

(5) It is very difficult to verify the sterilization procedure

(6) Personnel must be evacuated when steaming (long downtime)

(7) Substances that are highly toxic and classified as 'Class A' carcinogenic and cause cell variability

(8) Flammable

6.2 VHP compared to various types of sterilization methods - ClO2 and VHP

(1) Chlorine dioxide is generated from a chlorine gas container

(2) ClO2 is visible, yellow-green gas, gasification speed is very fast

(3) Compared with VHP, ClO2 is more toxic and has poor compatibility with materials.

6.3 VHP dry sterilization cycle

(1) Dehumidify dehumidification

(2) Condition is adjusted to a stable state

(3) Bio-decontamination disinfection

(4)Aerate into the filter to clean the air, in addition to VHP gas

6.4 Verification of VHP Sterilization

(1) The procedure for verifying the sterilization effect is the same as the verification of the moist heat sterilization cabinet.

(2) using biological indicator 1,0E+06 geobacillus Bacillus subtilis and stearothermophilus Bacillus stearothermophilus

(3) Sterilization cycle is about 2 hours, into VHP gassing 45 - 60 min, exhaust aeration 45 - 60 min

7, VHP sterilization technology summary

(1) It has been more than 15 years since VHP sterilization, and it is a very mature technology.

(2) sterilization time needs to see application settings

(3) The sterilization cycle with physical parameters and copyable with appropriate tools and biological indicators is verifiable

(4) So setting the sterilization cycle is easy - it is easy to verify

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