Shigella Castellani and Chalmers are Gram-negative bacilli, and their morphology is similar to that of
Other Enterobacteriaceae. No exercise. Facultative anaerobic, with both respiratory and fermentative types of metabolism. The catalase test was positive (with the exception of one species). Oxidase is negative. Organic can be nutritious. Fermented sugars do not produce gas (except for a few species of gas produced). Citrate or malonate is not used as the sole source of carbon. No growth in KCN, no production of H2S. It is an intestinal pathogen of humans and primates, causing bacterial dysentery.
The new national standard release of Shigella has caused us a lot of trouble. What should we do for anaerobic culture? Because we can do more, we can do 2-3 times a week, and 5-6 samples per day. . I took a day of anaerobic tank anaerobic tanks online, and finally got such a summary and shared it with the friends I need. Please bear with the wrong place.
one. 7L Japan Mitsubishi MGC AnaeroPack series anaerobic tank (ie anaerobic box)
Quote: 1200 yuan
Anaerobic bag: 298 yuan / 10 for each use
Indicator bar: 160 yuan / 10 each time with 1 reusable 3-4 times
Cost per time: 105.4 yuan
Its size is: 28*21.3*11.2
two. Kunke Instruments Quotes Japan Mitsubishi 7L
Price: 850 yuan - MGC 7.0L anaerobic culture box transparently placed 42 standard culture dishes
Anaerobic gas bag: 350 yuan / 10 each time - MGC C-35 anaerobic gas bag 3.5L (10 sachets / bag)
Anaerobic indicator: 100 yuan / 10 each time with 1 reusable 3-4 times - MGC C-22 oxygen indicator 10 sachets / bag
Cost per time: 80 yuan
In stock.
Supporting supplies:
AnaeroPack (peace package)
Anaerobic gas-producing bag: It is specially used for sealed cans of 3.5L or above. After the agent is sealed in the container, the oxygen in the container is completely absorbed within 0.5~1 hours, and the same amount of carbon dioxide is generated, which is suitable for anaerobic culture.
Micro-aerobic gas-producing bag: After the drug is sealed in a container, the oxygen in the container is absorbed in half within 0.5~1 hours, and the same amount of carbon dioxide is generated, and the concentration of O 2 and CO 2 becomes about 10%, which is suitable for micro-needs. Oxygen culture.
Medium storage bag: self-contained sealing strip, transparent appearance, easy to operate, easy to observe. Most suitable for the cultivation of a small number of samples.
CO 2 gas bag: After the drug is sealed in the container, the oxygen in the container is partially absorbed, the concentration is about 15%, and the carbon dioxide concentration is about 6%, which is suitable for the cultivation of carbon dioxide bacteria.
Oxygen indicator : anaerobic state (O 2 <0.1%): light purple to pink; aerobic state (O 2 >0.5%): light blue to dark blue. Used to detect the presence of oxygen, can be reused 4-5. Each indicator has a pinhole in the package, so it is not necessary to take out the granular indicator. The indicator granules must be stored frozen under sealed conditions.
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