Matters needing attention when using high pressure steam sterilizer

If the autoclave is used improperly, it will cause danger. In the experiment, we should pay attention to its use:

(1) The items to be sterilized should not be placed too tightly.

(2) The cold air must be completely excluded, otherwise the temperature in the pot will not reach the specified temperature, which will affect the sterilization effect.

(3) After the sterilization is completed, the gas can not be decompressed and decompressed, otherwise the liquid in the bottle will violently boil, and the bottle stopper will be washed out and overflow even causing the container to burst. The lid must be opened after the pressure in the sterilizer has fallen to the same level as the atmospheric pressure.

(4) The tampon of the test tube or bottle containing the medium should be coated with oil paper or kraft paper to prevent condensation from entering.

(5) In order to ensure the sterilization effect, the sterilization effect should be checked regularly. The usual method is to place the sulfur powder (melting point 115 ° C) or benzoic acid (melting point 120 ° C) in a test tube, and then carry out a sterilization test. If the above substances are melted, it means that the temperature in the autoclave has reached the requirement, and the sterilization effect is reliable. The adhesive paper for detecting the effect of the sterilizer (the temperature sensitive indicator thereon) can also be attached to the outer packaging of the article to be sterilized, such as the discoloration of the indicator on the adhesive tape, and the sterilization effect is reliable.

(6) Now there is a microcomputer-controlled high-pressure steam sterilizer. After the air conditioner is put off, the instrument can automatically press the constant voltage. When the time is up, the power is automatically cut off and the whistle is swayed, which is convenient to use.

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