Dual connector solution - Introduction to Solulink
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Solulink has established a proprietary, ready-to-use, catalytic linker technology that combines biomolecules in a highly efficient, high-yield, fast and quantitative manner. Coupling of biomolecules is the basis for ELISAs, affinity chromatography and particle analysis. However, standard maleimide, SMCC and EDAC coupling chemistry is slow, inefficient, time consuming, and difficult to detect is well known. Add unnecessary time and cost. At Solulink, we use our proprietary joint technology to change existing methods.
Defects of traditional dual-function connectors
Coupling is typically accomplished using a bifunctional linker, such as SMCC (succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate) which has a different reaction at each end to link the two molecules. One end (through the NHS ester) reacts with the amino group in the lysine and the N-terminus on the protein, and the other end (via the maleimide) reacts with the thiol group (-SH) on the cysteine. The flaws of this approach are often overlooked:
1. Undetectable - unable to quantify the conjugate formed
2. Having molecular weight dependence - coupling efficiency depends on the molecular weight of the two proteins
3. Low efficiency - post-reaction modification leads to the formation of some non-target copolymers
4. Unstable - short-term stable and easily hydrolyzed
5. Synthetic incompatibility - it is not possible to bind a linker to an oligonucleotide or peptide synthesis.
Solulink 's dual connector solution
Solulink's proprietary joint technology is a novel catalytic, UV-traceable linker that provides high efficiency and high yields of coupling in a fairly simple manner. Solulink introduces two types of linkers: HyNic (6-hydrazino-nicotinic acid, an aromatic hydrazine) and 4FB (4-formylbenzoate, an aromatic aldehyde), in the solution provided by Solulink, through proteins, oligonucleotides, carbohydrates Or the original amino group on the surface, molecule 1 is attached to HyNic, and molecule 2 is attached to 4FB. The mixing of the two molecules, through the TurboLink catalyst, aniline, allows the two linkers to react rapidly, selectively and efficiently. The result was coupled by a UV-tracked, bis-arylhydrazone, and the amount of conjugate formed was measured by absorbance at 354 nm. Any two proteins, oligonucleotides, polypeptides, etc., regardless of molecular weight, can be coupled efficiently.
Solulink 's dual connector advantage
1. Catalytic coupling - efficient and productive, faster kinetics
2. Quantitative - with a UV signal wavelength and simple UV scanning
3. Stable - its stability is 10 times that of other coupled joints
4. Water-soluble - "sulfo" variant avoids dangerous organic solvents such as DMF
5. Specificity - double joint method to avoid the formation of copolymer
6. Efficient - more than 80% coupling rate of the linker - biomolecule conjugate formation
7. Robust - the coupling bond is stable at 92 °C and pH 2.0-10.0
8. High yield - purity of 40-60% of starting biomolecules