Rat (PI) ELISA kit technology principle
If you purchase any of the company's enzyme-linked immunosorbent ELISA kits and ELISA kits, you only need to test the species (pig, big mouse, rabbit, human, chicken, cow, sheep, etc.) and test indicators. (Interleukins, hormone endocrine, interferon, growth factors, etc.) and the number of specimens (48T/96T) tell the company's sales staff to provide experimental free testing services. Product Name: Rat (PI) ELISA Kit Technical Principles English Name: Rat Proinsulin, PI ELISA Kit ELISA kit composition and reagent preparation (the following is the general instructions for ELISA kits, does not include special kits, please refer to the instructions for each product for specific instructions!) Bring your own items Collection and preservation of specimens If the experimental results are not satisfactory, please contact customer service or technical support in time, we can help you analyze the experimental results together. Pistol Box,Gun Pistol Safe,Biometric Pistol Safe,Fingerprint Handgun Safe Hebei Hupai Aodi Cabinet Industry Co.,Ltd. , https://www.hupaiaodi.com
Product Code: QY-SZ1984
Product traits: liquid product use: scientific research experiment product specifications: 96T/48T
Customer Service Phone QQ Email @qq.com
1. Enzyme plate: one piece (96 holes)
2. Standard (freeze-dried): 2 bottles, each bottle is diluted to 1ml with the sample dilution before use, and then allowed to stand for more than 10 minutes, then repeatedly reversed/twisted to help dissolve. The concentration is 1,600pg. /ml, after diluting it to 400 pg/ml, do a series of dilutions (note: do not directly dilute in the plate), diluted to 400 pg / ml, 200pg / ml, 100 pg / ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, the sample dilution was directly prepared as a standard concentration of 0 pg/ml, and was prepared within 15 minutes before use. For example, prepare 200 pg/ml standard: take 0.5ml (not less than 0.5ml) 400 pg/ml of the above standard into an Eppendorf tube containing 0.5ml of sample dilution, mix well, and so on.
3. Sample dilution: 1 x 20 ml.
4. Test dilution A: 1 x 10 ml.
5. Detect dilution B: 1 x 10 ml.
6. Detection solution A: 1 × 120μl (1:100) diluted with the test solution A 1:100 before use, prepared according to the total amount of each experiment required before dilution (100μl / hole), the actual More 0.1-0.2ml should be prepared during preparation. For example, 10 μl of test solution A plus 990 μl of test diluent A was prepared, gently mixed, and prepared within one hour before use.
7. Detection solution B: 1 × 120 μl / bottle (1: 100) diluted before use to detect dilution B 1:100. The dilution method is the same as the detection solution A.
8. Substrate solution: 1 x 10 ml / bottle.
9. Concentrated washing solution: 1 × 30ml / bottle, each bottle is diluted 25 times with distilled water.
10. Stop solution: 1 x 10 ml / bottle (2N H2SO4).
11. Lamination: 5 sheets
12. Instruction manual: 1 copy
1. Microplate reader (recommended to refer to the instrument instructions for preheating)
2. Micro dosing device and tip, EP tube
3. Distilled or deionized water, new filter paper
1. Serum: Whole blood samples should be placed at room temperature for 2 hours or 4 °C overnight, centrifuged at 1000 xg for 20 minutes, the supernatant can be detected, or the specimens should be stored at -20 ° C or -80 ° C, but should be avoided. Freezing and thawing.
2. Plasma: EDTA or heparin can be used as anticoagulant. Centrifuge at 2 - 8 ° C 1000 xg for 15 minutes within 30 minutes after collection, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing. melt.
3. Other biological specimens: centrifuge at 1000 xg for 20 minutes, take the supernatant for testing, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing and thawing.
4. Sample processing: Serum or plasma specimens are recommended to be diluted 5,000 times.
Note: The above specimens should be stored at 4 ° C for less than 1 week, -20 ° C or -80 ° C should be sealed and stored, -20 ° C should not exceed 1 month, -80 ° C should not exceed 2 months;
Rat (PI) ELISA kit technology principle Our products are provided with instructions, free of charge.
If it is not possible to judge by experimental data only, you can send the kit back to us for post-sale testing.
If it is the quality of the kit itself, you can return it for you;
If it is a problem with the experimental operation, the technician can give you some suggestions for improvement.
Tips: Not for clinical treatment.