Human polypeptide YY (Peptide-YY) ELISA kit instruction manual
Human polypeptide YY (Peptide-YY) ELISA kit instruction manual Product number: QY-KJ1217 Specification: 96T/48T Product Alias: Human Peptide YY (Peptide-YY) ELISA Quantification Kit Human Peptide YY (Peptide-YY) Enzyme-linked immunosorbent kit. Determination of serum or plasma, tissue and related liquid samples content or activity, size 96T / 48T, storage conditions: 2-8 ° C, valid: 6 months Tips: Not for clinical treatment. Transparent Dressing MDK-TD-01 China Transparent Dressing,Transparent Membrane Dressing supplier & manufacturer, offer low price, high quality Transparent Medical Dressing,Clear Surgical Dressing, etc. Transparent Dressing,Transparent Membrane Dressing,Transparent Medical Dressing,Clear Surgical Dressing Henan Maidingkang Medical Technology Co.,Ltd , https://www.mdkmedical.com
Technical principle:
(1). Solid phase of antigen or antibody and enzymatic labeling of antigen or antibody.
(2). The antigen or antibody bound to the surface of the solid support still retains its immunological activity.
(3). The enzyme-labeled antigen or antibody retains both its immunological activity and the activity of the enzyme.
(4). The specimen to be tested reacts with an antigen or antibody on the surface of the solid support. Further, an enzyme-labeled antigen or antibody is added, and is also bound to the solid phase carrier by a reaction.
(5). At this time, the amount of enzyme on the solid phase is proportional to the amount of the substance to be tested in the specimen.
(6) After adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the substance to be tested in the sample, so that qualitative or quantitative analysis can be performed according to the depth of the color. The assay has high sensitivity (pg-ng/ml level) and good repeatability. Based on the immunological reaction, a highly sensitive test technique combining the specific reaction of antigen and antibody with the efficient catalytic action of the enzyme on the substrate.
ELISA kit
1 30 times concentrated washing solution 20ml × 1 bottle; 2 enzyme standard reagent 6ml × 1 bottle
3 enzyme label coating plate 12 holes × 8; 4 sample dilution 6ml × 1 bottle
5 developer A solution 6ml × 1 bottle; 6 developer B solution 6ml × 1 bottle
7 Stop solution 6ml × 1 bottle; 8 standard (48ng / ml) 0.5ml × 1 bottle
9 standard dilution 1.5ml × 1 bottle; 10 instructions 1 copy
11 sheets of sealing film; 12 sealed bags 1
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