Single cell algae cultivation method (1)
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The cultivation process of the unicellular algae can be divided into four steps: disinfection of containers and tools, preparation of culture broth, inoculation and cultivation.
First, disinfection of containers and tools
1. Heat sterilization method This method is the use of high temperature to kill microorganisms. Containers and tools that cannot withstand high temperatures, such as plastics and rubber products, cannot be sterilized by heating.
(1) Direct Ignition Sterilization This method can directly burn microorganisms and sterilize them completely, but it is only suitable for disinfection of small metal or glass tools.
(2) Boiling disinfection Sterilize with water and boil for 5 minutes to 10 minutes. This is suitable for the disinfection of small containers and tools.
(3) Sterilization of drying ovens is also known as thermostatic oven sterilization.
2. Disinfection of chemicals In large-scale production culture, large containers, tools, FRP sinks, and concrete tanks are generally chemically disinfected.
(1) Alcohol with an alcohol concentration of 70% is commonly used for the disinfection of medium and small containers. Use gauze and alcohol to apply on the surface of the container and the tool. After 10 minutes, rinse it with sterile water twice. Alcohol is an ideal disinfectant.
(2) When potassium permanganate is disinfected, mix 300ppm of potassium permanganate solution, and put the cleaned container and tools in the solution for 5 minutes, remove, and then rinse with sterile water twice or three times.
Second, the preparation of culture fluid
The culture liquid (liquid medium) of the monocellular algae is prepared by adding various nutrients to the disinfected seawater (or fresh water).
1. Disinfection of seawater
(1) Heating and sterilizing method The seawater that is precipitated or precipitated and then sand-filtered is heated and sterilized in a flask or an aluminum pan. Generally, the temperature is increased to about 90 degrees Celsius and maintained for 5 minutes or until the boiling is reached. After the seawater is heated and sterilized, it is to be cooled. Before the fertilizer is added, it must be fully stirred so that the amount of dissolved gas which is reduced due to warming in the seawater can be restored to a normal level.
(2) Filtration and disinfection The precipitated seawater is filtered through a sand filter device to remove large organisms and non-living organisms, and then filtered through a ceramic filter tank to remove tiny organisms.
(3) Sodium hypochlorite disinfection method Adds 20ppm of sodium hypochlorite containing available chlorine per cubic meter of seawater, inflates for 10 minutes, stops the gas, after disinfection for 6 hours to 8 hours, 25 grams of sodium thiosulfate per cubic meter of water is added. Strong inflating 4 hours to 6 hours, then use sulfuric acid - potassium iodide - starch test solution to determine the presence of free chlorine in the sea can be used.
2. Preparation of the culture fluid According to the requirements of the cultivation of algae on the nutrition, the appropriate formulation is selected. (To be continued)