Cultivation of Pleurotus ostreatus
The species of parent strains, original species and cultivars. It is also commonly referred to as first-class, second-class, and third-class, and its cultivation method is different. The technical points are as follows: First, the parent species culture: the parent species is the use of tissue separation or spore isolation of bacteria. The mother culture medium is usually prepared using potato agar culture medium. Prepare 1000 ml of culture medium, 200 g potato, 18-20 g agar (garbage), and 20 g glucose. Method: Wash the potatoes and peel them, cut them into small pieces of broad bean, put in 1000 ml of water and boil for 30 minutes, and filter them with 6-8 layers of sand cloth to make up for the loss of moisture. After the agar was added to dissolve, dissolved sugar was added and water was added to 1000 ml. Afterwards, aliquots of test tubes will be dispensed in a quantity of 1/5-1/4 per tube, plus tampon and wrapper. Sterilize under a pressure of 1.2 kg for 30 minutes and place it in a sloping heat. After cooling, it is observed that no bacteria can be inoculated under a condition of 25-27°C. One female species can be piped 25-30. Aseptically inoculated tubes are incubated at a constant temperature of approximately 25°C, and the mycelium can be grown in vitro in approximately half a month. During the cultivation process, care should be taken to remove the contaminated test tubes. The cultivated mother species is transferred to the refrigerator and stored at 4°C for six months or at room temperature for three months. Second, the original species culture: the original species of raw materials can be used wood chips (sawdust) or cottonseed skin. Sawdust medium formulation: wood chips 78%, wheat bran or rice bran 20%, sugar 1%, gypsum 1% (both raw and cooked gypsum can be, but to be ground into powder can be applied). Cottonseed bark medium formulation: 100% cottonseed meal plus potato leaching juice 3%, (3 kilograms of potatoes cut into mud and boiled for 30 minutes filtrate), sugar 0.1%, magnesium sulfate 0.1% (one-thousandth of water). Method: Mix the water with a uniform mixture of materials. The humidity of the culture materials should be tightly grasped with your hands. See the water droplets in the fingers, and the drops are not suitable. The general ratio of water to water is 1:1.2-1.3 (ie, 100 kg of water is added to 120 -130 pounds). Then bottling, the bottling method is to gently shake the bottle while loading, so that the upper and lower parts of the bottle are close to the middle, and the shoulders of the bottle will be flattened, the culture materials inside and outside the bottle mouth will be cleaned, and the tampon is wrapped in paper. It is put into the autoclave for sterilization and sterilized under a pressure of 1.5 kg for one hour. After cooling, inoculate under sterile operating conditions. The test tube species is inserted into the bottle, one parent species can be connected to 4-5 bottles of the original species. After inoculation, the mycelium can be grown at a constant temperature of 25°C for about one month to grow the bottle. This is the original species. Third, cultivating species culture: The preparation of cultivars is actually a breeding expansion of the original species. The formulation of the cultivating seed wood culture medium is the same as that of the original seed culture. Cottonseed bark medium formula is also the same as the original culture formula. Bacteria are sterilized after bottling, and the original species of bacteria is inserted into the cultivating species bottle under aseptic conditions. A bottle of 750 ml of the original species can be connected to 80-100 bottles after cultivation. After inoculation, it is incubated at a constant temperature of 25°C for about one month, and it can be sown after being filled with the bottle. When cultivating primary species and cultivated species, inspect once every 2-3 days after inoculation until the mycelium is over the material surface and check once a week. Pick out bacterial strains that have been contaminated with bacteria and treat them away from the culture room. Surgical Gowns,Disposible Surgical Gowns,Anti-Virus Surgical Gowns,Medical Isolation Surgical Gowns Zhende Medical Co.,Ltd , https://www.zdmedicalproduct.com