Determination of green peel
Determination of content According to high performance liquid chromatography (Appendix VI D) determination. Chromatographic conditions and system suitability test octadecylsilane bonded silica gel as a filler; methanol - water (25:75) as the mobile phase; detection wavelength of 284nm. The number of theoretical plates should not be less than 1000 calculated according to the hesperidin peak. The preparation of the reference solution was accurately weighed to obtain the appropriate amount of the hesperidin reference substance, and methanol was added to prepare a solution containing 0.1 mg per 1 ml of the solution. Preparation of the test solution to take this product fine powder 0.2g, accurately weighed, set green bamboo 50ml volumetric flask, add methanol 30ml, ultrasonic treatment for 30 minutes, let cool, add methanol to the mark, shake, filtration, precision The amount of continuous filtrate 2ml, set 5ml volumetric flask, add methanol to the mark, shake, that is, too. The assay method accurately draws 1 μl of each of the reference solution and the test solution, and injects into the liquid chromatograph to measure and obtain it. This product contains hesperidin (C28H34O15) not less than 5.0%. Olive Net Problems,Olive Net Packages,Olive Net Network,Olive Harvest Net Changzhou Satidi Import and Export Co., Ltd. , https://www.czguanjiechuck.com