Carbon dioxide incubator pollution-free control technology

(Shanghai Ruisai Scientific Instrument Co., Ltd. Shi Guozhong Zhang Xiangyu Collection)

The carbon dioxide incubator simulates the growth environment of a similar cell / tissue in the living body such as a stable temperature ( 37 ° C ), a stable CO 2 level ( 5% ), and a constant pH ( pH : 7.2). -7.4 ), a higher relative humidity ( 95% ), a device for in vitro culture of cells / tissues.   Widely used in cell, tissue culture and culture of certain special microorganisms, commonly in cell dynamics research, collection of mammalian cell secretions, carcinogenic or toxicological effects of various physical and chemical factors, research and production of antigens, culture Hybridoma cells produce research fields such as antibodies, in vitro fertilization ( IVF ), stem cells, tissue engineering, drug screening, and the like.

  In living organisms, organisms have their own immune system to protect cells or tissues, but when cultured in vitro, there is no immune barrier to protect themselves. For the basic parameters of CO2 incubator temperature, CO2 and humidity, most incubators can meet the needs of research experiments. However, for the various pollution sources faced in the cultivation process, the control methods and effects of the various brands of dioxygen incubators are not the same, so the biggest threat in the in vitro culture of cells is actually a pollution problem.

Major sources of contamination in carbon dioxide incubators: bacteria, fungi (mold and yeast), viruses, mycoplasma, non-homologous cells.

       For cells, the ideal culture environment is also suitable for the survival of these pollutants. The incubator itself is not discernible, and most of the time in cell culture, the cells are located in the incubator, so whether the box can be inhibited Bacteria or sterilization is very important!

      Bacteria: After bacterial contamination, the medium will change color in 1-2 days, which has obvious influence on cell growth. The contaminated cells should be quickly isolated from other cell lines, discarded after sterilization, and disinfected with laboratory disinfectants and culture dishes. Clean platform.

      Virus: Due to the parasitic survival of the virus, it is isolated from the normal cells as soon as possible after the outbreak, and the disposal is relatively easy to handle, which poses a greater threat to the operator; although the virus-contaminated cells do not affect the primary culture, it is not safe to produce the vaccine. Therefore, the potential virus is a problem in the mass production of cells and the production of biological products such as vaccines and interferons.

Fungi (mold and yeast): Fungi grow slower, not as easy to detect as bacteria, but are contaminated once they are found; there are currently no good inhibitors, including the commonly used amphotericin, Once the pollution is easy to break out, especially the spores are hard to kill.

Mycoplasma: After mycoplasma contamination, because they do not cause cell death, they can coexist with cells for a long time. The medium generally does not turbid, the cells have no obvious changes, and the appearance gives a normal feeling. In fact, the cells have been affected by many aspects, such as causing Cell deformation, affecting DNA synthesis, and inhibiting cell growth are often overlooked by most experimenters.

Non-homologous cells: that is, cell cross-contamination, because the equipment and solutions required for each cell strain are not strictly separated during cell culture operation, one cell is often contaminated by another cell. At present, dozens of cells in the world have been contaminated by HeLa cells, causing many experiments to be invalid.

Therefore, once the above pollution occurs, the cells can only be discarded at the end, and the experiment cannot be saved. The experimental loss for many sampling difficulties cannot be estimated!

At present, the sterilization technology of the carbon dioxide incubator mainly includes dry heat, damp heat, ultraviolet lamp irradiation, disinfectant wiping, and the like.

High-temperature dry and hot air sterilization is recognized as the most effective sterilization technology in the world, which can completely eliminate all microbial pollution sources (including high temperature resistant Bacillus); moist heat sterilization is usually used in high pressure steam sterilization, less used in CO2 On the incubator, the moist heat sterilization is also called boiling method under normal pressure. The boiling water temperature is generally at least 100 °C , and the bacterial propagule is boiled for 5 minutes to be killed, but the spores often need to be boiled for more than 2 hours to be killed; The effectiveness of the UV lamp irradiation method is affected by many factors, such as occlusion, time, intensity, irradiation distance, humidity, etc., the sterilization effect is difficult to guarantee; the disinfectant wiping method is a method for sterilizing the surface, suitable for a flat surface such as The test bench, the dead space of the interior design of the cabinet, the various components can not be wiped and other factors lead to sterilization effect is very limited, and the chlorine, iodine disinfectant has a corrosive effect on stainless steel, can not wipe the stainless steel cabinet .

The UK LEEC TOUCH 190S CO2 incubator is a representative of a high-quality incubator. In terms of pollution-free control, it uses high- temperature dry-hot air sterilization technology up to 200 °C , and the entire sterilization process takes only 7 hours. Compared with other brands' sterilization process for more than ten hours, LEEC saves customers a lot of valuable experimental time while ensuring the complete elimination of all microbial pollution sources, and is increasingly favored by high-end customers.

In the design of the cabinet, the British LEEC TOUCH190S CO2 incubator has also been bacteriostatic design from many aspects: ( 1 ) the outer body is made of high quality stainless steel with antibacterial coating; ( 2 ) the inner wall is made of polished stainless steel, and the research shows polishing. Stainless steel can significantly reduce the growth of pollutants;   ( 3 ) Single-piece molding cavity, no welding, no gap, no dead angle; ( 4 ) HEPA high-efficiency air filter design can effectively filter out potential pollution sources and particulate impurities; ( 5 ) Fanless circulation, air Only by natural convection movement, there are no parts such as ventilation ducts that are difficult to remove and difficult to clean, which makes the cleaning work easier and eliminates a source of cross-contamination in cell culture.

Therefore, the UK LEEC TOUCH 190S CO2 incubator not only provides accurate in vitro simulation environment for cell growth, but also makes your cell culture safe and worry-free. It is the future development direction of cell culture incubator!  

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