**Porcine Reproductive and Respiratory Syndrome Virus Live Vaccine (CH-1R Strain)**
**1. High Safety**
The CH-1R strain used in the PRRS live vaccine is a weakened, non-pathogenic virus that ensures a high level of safety and stability. This makes it suitable for large-scale vaccination programs, including free vaccination campaigns.
To ensure its safety, two rigorous tests were conducted:
- **Toxicity Reversion Test:** Sows at 85–93 days of gestation, which are highly susceptible to PRRS, were inoculated with the vaccine. After 3–4 passages, no viral nucleic acid was detected in serum or nasal swabs, and no clinical signs of reproductive failure were observed. This indicates that the genetic stability of the CH-1R strain is well maintained.
- **Dose Safety Evaluation:** The vaccine was tested at different doses, including single and high-dose immunizations. Both pregnant sows and weaned piglets showed normal behavior, no adverse reactions, and normal litter sizes. These results confirm that the vaccine is safe for pigs of all ages.
**2. Strong Immune Efficacy**
The CH-1R strain is a domestic isolate of the American type, with over 98% homology to the Lanner disease isolate from 2006. This makes it highly effective against current circulating strains.
The vaccine provides rapid immunity, with protective antibodies detectable just one week after administration. It offers protection for up to two weeks, making it ideal for emergency use.
- **Vaccine Titer:** The vaccine has a titer above 10âµ.â° TCIDâ‚…â‚€ per head.
- **Immunity Duration:** Protection can last up to four months.
**Immunoprotection Test Results:**
- After immunizing 28–35-day-old piglets, IFA antibodies against PRRSV were detectable within 7 days, and 80% of the pigs were protected after two weeks.
- The minimum effective dose is 10â´.â° TCIDâ‚…â‚€ per head, but our factory standard is set at 10â¶.â° TCIDâ‚…â‚€ per head—100 times higher than the minimum.
- Long-term protection was confirmed by challenging pigs five months post-vaccination, with a 100% protection rate. For safety, the recommended immune period is set at four months.
**3. Low Immune Stress**
Pigs vaccinated with the CH-1R strain show no increase in body temperature, reduced feed intake, or weight loss. This indicates minimal stress on the animals, making it an ideal choice for commercial use.
---
**Porcine Reproductive and Respiratory Syndrome Inactivated Vaccine (CH-1a Strain)**
**Good Safety Profile**
As an inactivated vaccine, the CH-1a strain is inherently safer than live vaccines. It uses a domestically isolated strain (CH-1a) that has been passaged through Marc-145 cells, cloned, and purified. The final product is inactivated using chemical agents and combined with medical oil adjuvants.
When administered twice to pregnant sows and piglets, the vaccine caused only mild local reactions in some individuals, with no systemic side effects. The pigs remained active and had normal appetites, confirming the vaccine’s excellent safety profile. Additionally, because it is inactivated, it eliminates the risk of reversion to a virulent form, making it suitable for breeding pigs.
**Strong Immune Response**
The CH-1a strain induces strong and long-lasting immunity. After the second dose, pigs developed robust immunity within 20 days, with over 80% resistance to virulent challenges. The immunity lasts up to six months, making this vaccine a top choice for PRRS-negative farms.
**Protection for Newborn Piglets**
Sows vaccinated with the inactivated CH-1a strain transfer protective antibodies to their piglets through colostrum. This provides passive immunity during lactation. Studies have shown that maternal antibodies remain detectable in piglets as late as 24–28 days of age.
**Stability and Shelf Life**
The inactivated vaccine is stored at 2–8°C. When tested after 4, 8, and 12 months, the physical properties remained stable, and more than 90% of vaccinated pigs were protected when challenged with a virulent PRRS strain. This confirms the vaccine's long shelf life and consistent effectiveness.
This vaccine is a reliable and safe option for managing PRRS in both breeding and growing pigs.
DNA/RNA Purification Kit
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
Covid-19 Nucleic Acid Extraction Reagent,Nucleic Acid Extraction Reagent Kits,Dna Purification Kit,Rna Purification Kit
Jilin Sinoscience Technology Co. LTD , https://www.jlgkscience.com