Method for determining the germination rate of wheat
Brick Assay For this experiment, take two types of wheat seeds. Select an area of about 30 square centimeters with loose soil, free from pests, and ensure the soil is smooth and well-drained. Press bricks into the soil lightly to create a compacted surface. Place two seeds in the compacted area, then cover them with more bricks and water thoroughly. Each day, pour water over the bricks to maintain moisture. After 5 to 6 days, remove the bricks and observe the germination rate of the wheat seeds. Soil Determination Take 100 grains of each type of wheat seed. Choose fertile, loose soil that retains moisture well. Prepare a flat area of 30 square centimeters and dig two small furrows. Sow the two wheat varieties into the furrows and cover the soil gently. After 7 days, carefully check the seeds and calculate the germination rate based on the number of sprouted seeds. Fine Sand Measurement Use 50 grains of each type of wheat seed. Fill two pots with fine sand and spread the seeds evenly. Plant the seeds at a depth of approximately 3 cm, and keep the sand moist throughout the process. After 7 days, carefully dig up the seeds and determine the germination rate by counting the number of sprouted seeds. Toilet Paper Assay Take 50 grains of each type of wheat seed. First, wet the toilet paper and lay it flat in a container. Place the wheat seeds on the paper, then cover them with another layer of toilet paper. Ensure the paper remains moist daily. After 7 days, open the paper and count the number of germinated seeds to determine the germination rate. Wet Towel Assay Take 50 grains of each type of wheat seed. Soak the seeds in water for 24 hours before starting. Then place the seeds on two damp towels, roll the towels gently, and keep them moist every day. After 7 days, unfold the towels and examine the seeds to determine the germination rate. Rapid Assay Soak the wheat seeds in warm water (between 28°C and 30°C) for 3 to 5 hours to allow full hydration. Select representative seeds and cut them along the groove of the kernel using a blade, taking half of the embryo and discarding the other half. Place the cut seeds in a culture dish or porcelain bowl. Mix one part red ink with 19 parts fresh water to make a 5% red solution. Submerge the seeds in the solution for 5 to 10 minutes. Rinse the seeds thoroughly with clean water. Observe the embryos: if they are not stained or only slightly red, the seed is viable. If the embryo is fully red and the endosperm is also stained, the seed is no longer viable. Count the number of viable seeds and divide by the total number of tested seeds to determine the germination rate of the wheat. This set of experiments provides different methods to test the germination rate of wheat seeds, offering flexibility depending on the materials available. Whether using bricks, soil, sand, toilet paper, or a wet towel, each method helps evaluate seed viability effectively. The rapid assay, involving staining, offers a quick and visual way to assess seed health. These techniques can be used in educational settings, agricultural research, or home experiments to understand seed quality and improve planting success. Respiratory Infectious Bioantibody Biotechnology Co., Ltd. , https://www.bioantibodymedical.com